Julia Yaglom

Focus of Research:

Currently Attending:
Boston University

View more Publications by Julia Yaglom

HEAT SHOCK PROTEIN HSP72 CONTROLS ONCOGENE-INDUCED SENESCENCE PATHWAYS IN CANCER CELLS.

Co-Authors Gabai VL*, Waldman T, Sherman MY.

The heat shock protein Hsp72 is expressed at the elevated levels in various human tumors, and its levels often correlate with poor prognosis. Previously we reported that knockdown of Hsp72 in certain cancer cells but not in untransformed breast epithelial cells triggers senescence via p53-dependent and p53-independent mechanisms. Here we demonstrate that the p53-dependent pathway controlled by Hsp72 depends on the oncogenic form of PI3K. Indeed, upon expression of the oncogenic PI3K, epithelial cells began responding to Hsp72 depletion by activating the p53 pathway. Moreover, in cancer cell lines, activation of the p53 pathway caused by depletion of Hsp72 was dependent on oncogenes that activate the PI3K pathway. On the other hand, the p53-independent senescence pathway controlled by Hsp72 was associated with Ras oncogene. In this pathway, ERKs were critical for senescence, and Hsp72 controlled the ERK-activating kinase cascade at the level of Raf-1. Importantly, upon Ras expression, untransformed cells started responding to knockdown of Hsp72 by constitutive activation of ERKs, culminating in senescence. Therefore, Hsp72 is intimately involved in suppression of at least two separate senescence signaling pathways that are regulated by distinct oncogenes in transformed cells, which explains why cancer cells become "addicted" to this heat shock protein.

Mol Cell Biol. 2008 Nov 10. [Epub ahead of print]